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. 2016 Sep 12;12(9):e1006311. doi: 10.1371/journal.pgen.1006311

Fig 10. Diterpenoid phytoalexin biosynthesis is up-regulated in the lrd6-6 mutant.

Fig 10

(A) The geranylgeranyl diphosphate (GGDP) initiated synthesis pathway of diterpenoid phytoalexins. The key enzyme genes with increased expression detected by RNA-seq are shown in red. Data in the brackets are the gene expression fold-changed determined by qRT-PCR with gene specific primers (Fold-changed ± SD), asterisks denote statistical gene expression increases in lrd6-6 mutant compared to Kitaake by Student’s t-test (**, P < = 0.01). (B) Comparison of the expressions of OsMPK3, OsMPK4 and OsMPK6, which regulate synthesis of diterpenoid phytoalexins, between the lrd6-6 mutant and Kitaake. The expression levels of the genes were normalized to the Ubq5 reference gene. Error bars represent the SDs for three biology repeats and the expression differences was determined by Student’s t-test (**, P < = 0.01; N.D., No significantly difference). (C) Content determination of the phytoalexins as indicated in Kitaake and lrd6-6. The data was obtained from four biological replicates. The error bars indicate the SEMs. Asterisks denote a significant difference between the lrd6-6 mutant and Kitaake (Student’s t-test; **, P < 0.01).