Figure 3.
Immunochemical detection of 5-mC.
Notes: (A) Dot blot of genomic DNA isolated from nanoparticle-treated and -untreated cells and probed with anti-5-mC antibodies. Bound antibody was detected using a horseradish peroxidase-conjugated secondary antibody and chemiluminescence. (B) Methylation status of genomic DNA cytosine methylation in TiO2-treated cells. Detection of 5-mC present in the genomic DNA of control and TiO2-treated cells. (C) Methylation status of genomic DNA cytosine methylation in ZnO-treated cells. Detection of 5-mC present in the genomic DNA of control and ZnO-treated cells. Methylation was estimated by an enzyme-linked immunosorbent assay based Imprint® Methylated DNA Quantification Kit (Sigma-Aldrich), which specifically detects 5-mC in the input DNA. Data are represented as percent cytosine methylation as compared with control. One-way analysis of variance (P<0.05) shows statistically significant difference.
Abbreviations: 5-mC, 5-methylcytosine; TiO2, titanium dioxide; ZnO, zinc oxide.