Figure 2.

OVA‐specific transferred T cells are responsive to oral tolerance in TLR2^−/− recipient mice. (a) Schematic of methods for adoptive transfer and assessment of Tregs in OVA tolerance. 1 × 10⁶ naïve CD62L⁺/CD4⁺ cells were purified by MACS from OT‐II ⁺/CD45.1⁺/Foxp3‐GFP mice and adoptively transferred into naïve C57BL/6 or TLR2^−/− recipient mice by i.v. injection. Mice were provided with 4 mg/mL OVA in water ad libitum, while control mice were provided with normal water (not depicted). On day 7, the PPs, MLN, and spleen were harvested and assessed for recovered OT‐II ⁺/CD4⁺/CD45.1⁺ cells by flow cytometry. (b) Representative plots of transferred cells recovered from the MLN outlining gating strategy for analysis of flow cytometry. (c), Transferred OT‐II Treg cells recovered from the MLNs, spleens (d), and PPs (e) were compared between OVA‐tolerized and untreated C57BL/6 or TLR2^−/− mice by t‐tests. Bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.