Figure 5.

Antigen‐specific Treg levels are not altered in vivo by oral Pam₃ CSK ₄ treatment. (a) Schematic of methods for adoptive transfer and assessment of Tregs in OVA tolerance. 1 × 10⁶ naïve CD62L⁺/CD4⁺ cells were purified by MACS from OT‐II ⁺/CD45.1⁺/Foxp3‐GFP mice and adoptively transferred into naïve C57BL/6 or TLR2^−/− recipient mice by i.v. injection. Mice were treated with OVA ad libitum and supplemented with 3 gavage treatments of OVA ± Pam₃ CSK ₄. On day 7, the PPs, MLN, and spleen were harvested and assessed for recovered OT‐II ⁺/CD4⁺/CD45.1⁺ cells by flow cytometry. (b) Transferred OT‐II Treg cells recovered from the MLNs, spleens (c), and PPs (d) were compared by anova followed by Bonferroni's multiple comparison test. Bars represent mean ± SEM. n.s., not significant.