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. Author manuscript; available in PMC: 2017 Feb 18.
Published in final edited form as: Nature. 2016 Aug 18;536(7616):304–308. doi: 10.1038/nature19071

Figure 1. Reconstituting E2Ubc9-SUMOD/E3Siz1-SUMOB/PCNA.

Figure 1

a, Schematic for nucleophilic attack of the E2-SUMO thioester by lysine or EDT modified cysteine with in vitro SUMO modification of PCNA. b, Schematic of the tetrahedral intermediate during transthioesterification and EDT cross-linking of E2Ubc9A129K-SUMO and PCNA. c, Structure of the complex. d, Cartoon model of the complex. For gel source data, see Supplementary Figure 1.