Extended Data Table 2. Summary of Kinetic Constants For Multiple Turnover Experiments with Purified E2Ubc9-SUMOD68R Thioester.
Sizl Isoform | Additional SUMO Isoform | Km (µM) | kcat (s−1) | kcat/ Km (M−1/s−1) |
167–465 | - | 1.07 ± 0.10 | 1.27 ± 0.05 | 1.18 × 106 ± 0.12 × 106 |
167–449 | - | 1.23 ± 0.19 | 0.88 ± 0.05 | 7.15 × 105 ± 0.12 × 105 |
167–449 | Δ18Smt3ΔGGHIS | 1.84 ± 0.31 | 0.86 ± 0.06 | 4.68 × 105 ± 0.09 × 105 |
167–449 | Δ18Smt3D68RΔGGHIS | 1.08 ± 0.12 | 0.81 ± 0.03 | 7.49 × 105 ± 0.09 × 105 |
167–449-Δ18Smt3ΔGGHIS | - | 0.48 ± 0.15 | 0.64 ± 0.06 | 1.32 × 106 ± 0.43 × 106 |
167–449-Δ18Smt3D68RΔGGHIS | - | 4.40 ± 1.44 | 0.93 ± 0.18 | 2.11 × 105 ± 0.08 × 105 |
167–508 | - | 2.34 ± 0.58 | 0.54 ± 0.06 | 2.31 × 105 ± 0.06 × 105 |
167–508 | Δ18Smt3ΔGGHIS | 1.15 ± 0.14 | 0.89 ± 0.04 | 7.71 × 105 ± 0.10 × 105 |
167–508 | Δ18Smt3D68RΔGGHIS | 1.46 ± 0.18 | 0.75 ± 0.04 | 5.13 × 105 ± 0.07 × 105 |
167–508-Δ18Smt3ΔGGHIS | - | 0.29 ± 0.07 | 0.71 ± 0.04 | 2.43 × 106 ± 0.57 × 106 |
167–508-Δ18Smt3D68RΔGGHIS | - | 2.07 ± 0.29 | 0.70 ± 0.04 | 3.38 × 105 ± 0.05 × 105 |
Sizl Isoform | Additional SUMO Isoform | Km (µM) | kcat (s−1) | kcat/ Km (M−1/s−1) |
167–465 | - | 0.70 ± 0.07 | 4.26 ± 0.14 | 6.09 × 106 ± 0.69 × 106 |
167–449 | - | 0.91 ± 0.12 | 2.52 ± 0.12 | 2.76 × 106 ± 0.39 × 106 |
167–449 | Δ18Smt3ΔGGHIS | 1.44 ± 0.21 | 2.47 ± 0.15 | 1.71 × 106 ± 0.27 × 106 |
167–449 | Δ18Smt3D68RΔGGHIS | 0.90 ± 0.07 | 2.45 ± 0.07 | 2.71 × 106 ± 0.22 × 106 |
167–449-Δ18Smt3ΔGGHIS | - | 0.23 ± 0.05 | 1.47 ± 0.08 | 6.48 × 106 ± 1.58 × 106 |
167–449-Δ18Smt3D68RΔGGHIS | - | 1.02 ± 0.19 | 1.29 ± 0.09 | 1.26 × 106 ± 0.25 × 106 |
167–508 | - | 0.91 ± 0.12 | 1.12 ± 0.05 | 1.29 × 106 ± 0.18 × 106 |
167–508 | Δ18Smt3ΔGGHIS | 0.62 ± 0.07 | 2.01 ± 0.07 | 3.22 × 106 ± 0.39 × 106 |
167–508 | Δ18Smt3D68RΔGGHIS | 0.65 ± 0.10 | 1.47 ± 0.08 | 2.27 × 106 ± 0.36 × 106 |
167–508-Δ18Smt3ΔGGHIS | - | 0.14 ± 0.03 | 1.77 ± 0.08 | 13.09 × 106 ± 2.81 × 106 |
167–508-Δ18Smt3D68RΔGGHIS | - | 0.76 ± 0.10 | 1.34 ± 0.06 | 1.77 × 106 ± 0.24 × 106 |