Figure 1.

Mouse pluripotent stem cell cultures were assayed for relative expression levels of pluripotency (Nanog, SOX2), endodermal (SOX17, Pdx1), and pancreatic (Ins-1, Ins-2, and Gcg) genes at different points in time. RT-qPCR was performed in triplicate for each sample with bars representing means ± SD of 3 biological replicates. Expression levels were normalized against the housekeeping gene ppia. Asterisks (∗) denote statistical significant (P < 0.001) increases in pancreatic marker gene expression at specific time points of cultures as compared to nondifferentiated mouse pluripotent stem cells (day 0 of differentiation), # UD represents the undetected genes.