Table 1.

Nucleotide sequences of primer pairs used for real-time qPCR.

Gene	Forward 5′-3′	Reverse 5′-3′
Nanog	TCTCTCAGGCCCAGCTGTGT	CGCTTGCACTTCATCCTTTG
SOX2	AACCGATGCACCGCTACG	TTGACCACAGAGCCCATGG
SOX17	CTTCCCTACCAGGGACACGA	ACTGCTTCTGGCCCTCAGGT
Pdx1	GGTGCTTACACAGCGGAACC	CGGTCAAGTTCAACATCACTGC
Insulin-1	ACAGCATCTTTGTGGTCCCC	CAGCACTGATCCACAATGCC
Insulin-2	GCTCTTCCTCTGGGAGTCCC	AAGGTCTGAAGGTCACCTGCTC
Glucagon	GCCACTCACAGGGCACATT	GTCCCTTCAGCATGCCTCTC
ppia	CCAGGATTCATGTGCCAGG	GCCATCCAGCCATTCAGTCT