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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Virology. 2016 Jan 21;490:6–16. doi: 10.1016/j.virol.2015.10.013

Fig. 2.

Fig. 2

HIV Gag-iCre reporter signal is specific to fusion. (A) Jurkat floxRG target cells are transduced with the MSCV floxR-G cassette that expresses dsRed (upper) and switches to GFP expression upon fusion by recombination by Cre. (B) Fluorescent microscopy of infection of RG Jurkats incubated with Gag-iCre in the presence of fusion inhibitor (AMD3100), reverse transcription inhibitor (AZT) or untreated. (C) Red channel fluorescence of samples shown in C. (D) FACS analysis of RG Jurkats exposed to HIV Gag-iCre in the presence and absence of inhibitors.