Fig. 5.

Small molecule library screening overview. (A) Nucleofected Gag-iCre donor cells were co-cultured with Jurkat floxRG target cell line in the presence of each of the 1998 library compounds, performed in duplicate. Plates were incubated for 48 h followed by fixation and quantitation of fusion by flow cytometry. (B) Percent inhibition was calculated by normalizing to DMSO treated and uninfected controls. All compounds that inhibited fusion by 30% or more were considered first round hits.