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Western blot analysis of membrane and soluble fractions of E. coli cells expressing C-terminal His-tagged RnfB, using a six-His tag antibody: lane 1, 30 μg of membrane protein; lane 2, 10 μg of membrane protein; lane 3, 30 μg of protein from the soluble cell fraction; lane 4, 10 μg of protein from the soluble cell fraction. The arrow points to bands at approximately 20 kDa, the expected molecular mass of RnfB.
