Table 2.

strain or plasmid	(A) Strains and Plasmids Used in This Study genotype or description	source or reference
strain E. coli Top 10	F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZ M15 ΔlacX74 recAl araD139 A(ara-leu)7697 galU galK rpsL (Strr) endAl nupG	Invitrogen
E. coli Top 10 Δrnf		this study
E. coli LMG194	F− AlacX74 galE thi rpsL ΔphoA (Pvull) Aara714 leu::TnlO	Invitrogen
V. cholerae 0395N1	Smr	32
V. cholerae 0395 INI Δnqr	Smr	26
plasmid
pBAD-PhoA	pBAD-22 derivative containing signal sequence-less phoA with a 5′ Kpnl site	33
pBAD-GFP	pBAD plasmid with cycle 3 grp	34
ARANC	pBAD-PhoA rnfA; full-length C-terminal fusion	this study
ARBNC	pBAD-PhoA rnfB; full-length C-terminal fusion	this study
ARDNC	pBAD-PhoA rnfD; full-length C-terminal fusion	this study
ARDN92	pBAD-PhoA rnfD; C-terminal fusion at residue 92	this study
ARDN266	pBAD-PhoA rnfD; C-terminal fusion at residue 266	this study
ARGNC	pBAD-PhoA rnfG; full-length C-terminal fusion	this study
GRCNC	pBAD-GFP rnfC; full-length C-terminal fusion	this study
GRDNC	pBAD-GFP rnfD; full-length C-terminal fusion	this study
GRENC	pBAD-GFP rnfE; fiall-length C-terminal fusion	this study
GRGNC	pBAD-GFP rnfG; full-length C-terminal fusion	this study

	(B) Primers Used in This Study
fusion construct	forward 5′ → 3′'	reverse 5′ → 3′
ARANC	GGTACCGTTATTGCTTTGGCAAAGCAGG	GGTACCTTCAGTTTCACCAATCCGGTAAAG
ARBNC	GGTACCGAGTACCATAGTCATCGCTG	GGTACCTTATTCGCACTTTTCTGCGC
ARDNC	GGTACCGGCCTTCTTTATTGCTAGCTC	GGTACCTTGTGTCCGTAAGTTCTCGG
ARDN92	GGTACCGGCCTTCTTTATTGCTAGCTC	GGTACCAAGAGGGGCGGGATAG
ARDN266	GGTACCGGCCTTCTTTATTGCTAGCTC	GGTACCAAGGTTGTTCCCGGTGC
ARGNC	GGTACCGCTGACAGCAATTCGAAAAAATG	GGTACCTTTTGACCCTCACACGGATTC
GRCNC	GCTAGCTTGTCATTAATCGAACAAATCAAATC	GCTAGCGTCTTTCTCCTCAGCGTTTAA
GRDNC	ACTAGTGCCTTCTTTATTGCTAGCTCCC	ACTAGTGTGTCCGTAAGTTCTCGGTTTG
GRENC	GCTAGCAATGAAAACCGAACTCTGATGTTAAATGGC	GCTAGCGACATTGGTCACGCGTGC
GRGNC RnfB	GTAGCCTGACAGCAATTCGAAAAAATGG	GCTAGCTTGACCCTCACACGGATTC
RnfBEc	GCTAGAATTCGAGGAAATAATAAATGAATGCTATCTGGATTGCCG	AGTCAAGCTTTCAATGGTGATGGTGATGACCTTCAAGCTCGCCCTTAGCATGGTGTTCCACGG