Table 5.
Location of Functionally Important Residues in Rnf
| subunit | |
|---|---|
| RnfA | conserved acidic residues |
| E109, end of helix 3 | |
| RnfB | three 4Fe-4S centers |
| C49-C52-C57-C73 | |
| C114-C117-C120-C124 | |
| C144-C147-C150-C154 | |
| RnfC | NADH binding site |
| G18, G19, I20, I21, A174, E175, C176, E177, P178 | |
| three 4Fe-4S centers | |
| C405-C408-C411-C415 | |
| C378-C381-C384-C388 | |
| C417-C420-C423-C427 | |
| RnfD | FMN binding site |
| T184, M185, A186, T187,a periplasmic region between helices 5 and 6 |
|
| conserved acidic residues | |
| E220, helix 6 | |
| D288, helix 8 | |
| D321, helix 10 | |
| D338, helix 10 | |
| RnfG | FMN binding site |
| T172, G173, A174, T175,a periplasmic region after helix 1 | |
| RnfE | conserved acidic residues |
| E116, helix 4 | |
| D130, cytoplasmic region between helices 4 and 5 | |
| E150, helix 5 |
a
Ligand for the covalently bound FMNs.