. Author manuscript; available in PMC: 2016 Sep 12.

Published in final edited form as: Biochemistry. 2014 Dec 22;54(2):490–496. doi: 10.1021/bi501266e

Table 1.

Ubiquinone Reductase (CoQ-1 red) Activity of NqrB-D397 Mutants in the Absence of Cations and in the Presence of Saturating Concentrations^a of Na⁺, Li⁺, and K⁺^b

	CoQ red (s⁻¹)

enzyme	no cation	NaCl	LiCl	KCl
wild type	66.3	528.5	205.5	62.4
NqrB-D397C	61.9 ± 8.7	162.2 ± 12.3	110.6 ± 5.9	62.1 ± 10.3
NqrB-D397E	66.0 ± 4.8	129.6 ± 8.8	96.4 ± 10.3	67.4 ± 10.1
NqrB-D397N	59.7 ± 2.5	122.9 ± 4.3	95.5 ± 6.2	97.0 ± 7.4^c
NqrB-D397S	57.7 ± 4.1	101.3 ± 8.3	80.7 ± 5.0	60.3 ± 2.8

In 100 mM NaCl, LiCl, or KCl.

All experiments were repeated at least seven times with three independent preparations. The average and standard deviation are presented.

p < 0.05.