. Author manuscript; available in PMC: 2016 Sep 12.

Published in final edited form as: Biochemistry. 2014 Dec 22;54(2):490–496. doi: 10.1021/bi501266e

Table 3.

Rate Constants of the Reduction of NqrB-D397N by NADH in the Presence of Different Concentrations of KCl^a

	rate (s⁻¹)

[KCl] (mM)	FAD → FADH₂	RibH^• → RibH₂	FMN_c^•− → FMN_cH₂
0	321 ± 2.3	12.1 ± 1.6	1.85 ± 0.34
25	325 ± 3.5	15 ± 3.2	2.13 ± 0.48
50	312 ± 10.4	17 ± 0.55	2.6 ± 0.56
200	336 ± 7.7	21.5 ± 4.6	3.2 ± 0.78
300	331 ± 5.6	23 ± 8.2	3.7 ± 0.36
400	334 ± 2.3	23.7 ± 5.3	3.9 ± 1.2

Each experiment corresponds to at least 12 kinetic traces. Three independent experiments were averaged (corresponding to at least 36 individual traces).