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. Author manuscript; available in PMC: 2016 Sep 13.
Published in final edited form as: JACC Cardiovasc Imaging. 2015 Jul 22;8(8):873–884. doi: 10.1016/j.jcmg.2015.04.016

Figure 3.

Figure 3

Apoptosis detected after radiation exposure. (A) Sample flow cytometry dot plot from a patient after cardiac CTA. In order to identify apoptotic cells, lymphocytes were first isolated using forward and side scatter profiles (left). CD3+ T cells were then identified from within this subset (middle). Lastly, apoptotic T cells were detected using Annexin V, an early marker of apoptosis, and DAPI, an intracellular marker for dead cells, which are either necrotic or at the later stages of apoptosis (right). The estimate of apoptotic cell death includes the sum of cells that express Annexin only or Annexin-DAPI. This patient had 1.8% of apoptotic cells that express Annexin only and 0.2% of dead cells that express both Annexin and DAPI. (B) Scatterplot graph of the correlation analysis between the estimates of apoptosis and DNA damage (n=25). (C) Scatter plot graph of the disappearance of the phosphorylated ATM (pATM) over time as damaged cells are repaired or eliminated (n=25). (D) Bar graph displaying the relative percentages of cells that were repaired or had undergone programmed cell death in individual patients with evidence of cell death by flow cytometry (n=15). The remainder of the patients had no cell death and complete repair (n=10) (not shown in graph).