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Isolation of side population cells. A single cell suspension is isolated and stained with Hoechst 33342. A small percentage of cells are able to extrude Hoechst 33342 out of the cytoplasm through ABC transporters. To identify side population cells, the suspension is analyzed on a flow cytometer (Goodell et al., 1996). The side population cells (red gate) appear to the left side of the main population of cells.
