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. 2016 Sep 13;7:1367. doi: 10.3389/fpls.2016.01367

FIGURE 2.

FIGURE 2

Schematic representation of the CsLCYb1 promoter::GUS vectors construction. These constructs are based on the pCAMBIA1301 vector. LB, left border; 35S PolyA, Cauliflower Mosaic Virus 35S terminator; Hyg, hygromycin resistance gene; 35S P, Cauliflower Mosaic Virus 35S promoter; GUS, β-glucuronidase reporter gene; Nos PolyA, nopaline synthase terminator; RB, right border. Hollow arrows indicate the positions of the promoter insertion in the vectors. The promoters contain the full-length sequence (LP) and its five 5′ truncated fragments (LP1, LP2, LP3, LP4, and LP5). Numbers indicate the sequence length from the first base of the ATG.