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. 2016 Sep 13;7:1367. doi: 10.3389/fpls.2016.01367

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Copyright © 2016 Lu, Zhang, Zheng, Zhu, Xu and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GUS assays of transgenic citrus callus. (Up) Qualitative GUS staining. Citrus callus carrying different promoter constructs were separately subjected to histochemical GUS staining. Transgenic lines carrying the GUS reporter gene under the control of the CaMV35S promoter were used as the positive control (35S) and untransformed callus was used as the negative control (WT). (Down) Quantitative GUS assays of different promoter deletions in stably transformed citrus callus under various treatments, including abscisic acid (ABA), auxin (IAA), gibberellin (GA), salicylic acid (SA), Methyl Jasmonate (JA), Kinetin (KT), sucrose (Suc), glucose (Glu), and NaCl. Data are means ± SD of three independent experiments. Significant differences between values are indicated by asterisk (^∗P < 0.05, ^∗∗P < 0.01).