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. 2016 Sep 13;7:1439. doi: 10.3389/fmicb.2016.01439

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Copyright © 2016 Teixeira-Santos, Ricardo, Branco, Azevedo, Rodrigues and Pina-Vaz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of liposomal amphotericin B (L-AMB) in combination with colistin upon C. albicans (strain 596) physiological parameters. (A) Viability assessment by colony forming units (CFU) enumeration of C. albicans cells exposed to () L-AMB, () CST, and () L-AMB in association with CST. The CFU counts of cells non-treated are represented as (). (B) Cell membrane potential was evaluated using DiBAC₄(3) staining. (C) Cell membrane integrity was evaluated using propidium iodide (PI) staining. (D) Cell metabolic activity was evaluated using 5-CFDA staining. Data at respective time points corresponds to mean ± standard deviation. ^∗p-values < 0.05, significant differences between the treatments with L-AMB alone and CST alone vs. the control (non-treated cells); and between the association L-AMB/CST and L-AMB alone.

Inline graphic — Effect of liposomal amphotericin B (L-AMB) in combination with colistin upon C. albicans (strain 596) physiological parameters. (A) Viability assessment by colony forming units (CFU) enumeration of C. albicans cells exposed to () L-AMB, () CST, and () L-AMB in association with CST. The CFU counts of cells non-treated are represented as (). (B) Cell membrane potential was evaluated using DiBAC₄(3) staining. (C) Cell membrane integrity was evaluated using propidium iodide (PI) staining. (D) Cell metabolic activity was evaluated using 5-CFDA staining. Data at respective time points corresponds to mean ± standard deviation. ^∗p-values < 0.05, significant differences between the treatments with L-AMB alone and CST alone vs. the control (non-treated cells); and between the association L-AMB/CST and L-AMB alone.