Table 1.
Lpp of S. aureus USA300.
| No | Locus tag | Function/Annotation | PFAM | SP (aa) | Lipobox | Mass (KDa) | Disseminationa | References |
|---|---|---|---|---|---|---|---|---|
| Fe transport | ||||||||
| 01 | SAUSA300_1978 | Ferric hydroxamate receptor/FhuD1 | Peripla_BP_2 | 17 | LTA C | 34 | 15* | Sebulsky and Heinrichs, 2001; Sebulsky et al., 2004 |
| 02 | SAUSA300_2235 | Fe ABC transporter/FhuD2 | Peripla_BP_2, ABC2_membrane_3 | 17 | LAA C | 34 | 16 | Sebulsky and Heinrichs, 2001; Sebulsky et al., 2004; Mariotti et al., 2013 |
| 03 | SAUSA300_0721 | Transferrin receptor/SstD | Peripla_BP_2 | 18 | LAA C | 38 | 14 | Morrissey et al., 2000 |
| 04 | SAUSA300_0117 | Fe ABC transporter/SirA | Peripla_BP_2 | 20 | LAG C | 37 | 10 | Heinrichs et al., 1999 |
| 05 | SAUSA300_1032 | Fe ABC transporter/IsdE | Peripla_BP_2 | 19 | LTS C | 32 | 10 | Mazmanian et al., 2002, 2003; Grigg et al., 2007 |
| 06 | SAUSA300_0344 | FepA, Fe-binding protein, part of fepABC and tat-AC cluster | Peptidase_M75 | 17 | IAA C | 32 | 10 | Biswas et al., 2009 |
| 07 | SAUSA300_2136 | Fe ABC transporter | Peripla_BP_2 | 21 | VAA C | 36 | 14 | |
| 08 | SAUSA300_0219 | Iron Binding Protein | SBP_bac_1, 6, 8, 11 | 17 | LSA C | 36 | 4* | |
| Other cation transport | ||||||||
| 09 | SAUSA300_0618 | Manganese-binding protein MntC (SitC) | ZnuA, Nit_Regul_Hom | 17 | VAA C | 34 | 19 | Cockayne et al., 1998; Müller et al., 2010; Diep et al., 2014 |
| 10 | SAUSA300_2351 | Zinc-binding, adcA-like | ZnuA, ZinT | 20 | LAA C | 57 | 10 | |
| 11 | SAUSA300_2411 | Cobalt and nickel transporter Cnt (Opp1A) | SBP_bac_5 | 20 | LTG C | 59 | 10 | Remy et al., 2013 |
| 12 | SAUSA300_0231 | Nickel ABC transporter | SBP_bac_5 | 18 | LSG C | 55 | 10* | |
| 13 | SAUSA300_0203 | Nickel-Peptide/transporter substrate-binding protein | SBP_bac_5 | 18 | LSG C | 66 | + | |
| 14 | SAUSA300_2230 | Molybdenum ABC transporter (ModA) | SBP_bac_11, 1, PBP_like_2 | 19 | LAG C | 29 | 15 | Neubauer et al., 1999 |
| Anion transport | ||||||||
| 15 | SAUSA300_1283 | Phosphate ABC transporter | PBP_like_2, PBP_like | 20 | LGA C | 36 | 15 | |
| 16 | SAUSA300_0145 | Phosphonate ABC transporter | Phosphonate-bd, SBP_bac_3 | 20 | AAA C | 35 | 10 | |
| 17 | SAUSA300_0175 | Nitrate ABC transporter substrate-binding protein | NMT1_2, | 17 | ITG C | 36 | 4 | |
| AA and Peptide transport | ||||||||
| 18 | SAUSA300_2391 | Glycine betaine /carnitine/ choline ABC transporter (OpuCc) | OpuAC | 20 | LSG C | 37 | 19 | |
| 19 | SAUSA300_2359 | Amino acid ABC transporter | SBP_bac_3 | 17 | LAA C | 13 | 12 | |
| 20 | SAUSA300_0073 | Peptide ABC transporter | SBP_bac_5 | 19 | LAG C | 57 | 11 | |
| 21 | SAUSA300_0891 | Oligopeptide ABC transporter (Opp3A) | SBP_bac_5 | 20 | LSG C | 61 | 11 | Hiron et al., 2007 |
| 22 | SAUSA300_0892 | Oligopeptide ABC transporter (Opp4A) | SBP_bac_5 | 20 | LSA C | 63 | 5 | Hiron et al., 2007 |
| 23 | SAUSA300_0437 | NLPA/ D-Methionine binding (GmpC) | Lipoprotein_9 (NLPA) | 17 | LAA C | 31 | 9 | Williams et al., 2004 |
| 24 | SAUSA300_0798 | D-Methionine ABC transporter | OpuAC, Lipoprotein_9 | 19 | LAA C | 30 | 15 | |
| Sugar transport | ||||||||
| 25 | SAUSA300_0209 | Maltose ABC transporter | SBP_bac_1, 8 | 20 | VTA C | 47 | 6 | |
| Miscellaneous functions | ||||||||
| Biosynthesis | ||||||||
| 26 | SAUSA300_1884 | CamS sex pheromone biosynthesis | CamS | 17 | LAA C | 44 | 14 | |
| Respiration | ||||||||
| 27 | SAUSA300_0963 | Quinol oxidase, subunit II (QoxA) | COX2 | 19 | LSG C | 41 | 21 | |
| 28 | SAUSA300_0693 | Electron transfer domain/SaeP | CfAFP, DM13 | 20 | LGA C | 16 | 22 | Makgotlho et al., 2013 |
| Chaperone-Foldases | ||||||||
| 29 | SAUSA300_1790 | Foldase protein PrsA | Rotamase, | 20 | LGA C | 36 | 15 | Heikkinen et al., 2009; Jousselin et al., 2012 |
| 30 | SAUSA300_2354 | Thioredoxin/Protein disulfide-isomerase | Thioredoxin_2, 4, 5 | 18 | LTA C | 22 | 15* | |
| Protein translocation | ||||||||
| 31 | SAUSA300_2046 | YidC (OxaA)–essential protein | OATP, 60KD_IMP | 19 | LAG C | 32 | 25 | |
| Phage and plasmid encoded Lpp | ||||||||
| 32 | SAUSA300_1436 | PhiSLT ORF144-like | DUF1510, Zip, Presenilin | 17 | LTA C | 16 | 2* | |
| 33 | pUSA300_HOUMR0011 | Membrane bound penicillinase BlaZ | 16 | LSA C | 31 | 11 | Nielsen and Lampen, 1982b | |
| Lpl cluster | ||||||||
| 34 | SAUSA300_0410 | Lpl-1 νSaα specific | DUF576 | 32 | IAG C | 30 | + | Nguyen et al., 2015b |
| 35 | SAUSA300_0411 | Lpl-2 νSaα specific | DUF576 | 22 | IIG C | 30 | + | Nguyen et al., 2015b |
| 36 | SAUSA300_0413 | Lpl-3 νSaα specific | DUF576 | 23 | IIG C | 30 | + | Nguyen et al., 2015b |
| 37 | SAUSA300_0414 | Lpl-4 νSaα specific | DUF576 | 22 | VTS C | 28 | + | Nguyen et al., 2015b |
| 38 | SAUSA300_0415 | Lpl-5 νSaα specific | DUF576 | 22 | IMG C | 29 | + | Nguyen et al., 2015b |
| 39 | SAUSA300_0416 | Lpl-6 νSaα specific | DUF576 | 20 | MAG C | 29 | + | Nguyen et al., 2015b |
| 40 | SAUSA300_0417 | Lpl-7 νSaα specific | DUF576 | 23 | IVG C | 30 | + | Nguyen et al., 2015b |
| 41 | SAUSA300_0418 | Lpl-8 νSaα specific | DUF576 | 22 | VTS C | 29 | + | Nguyen et al., 2015b |
| 42 | SAUSA300_0419 | Lpl-9 νSaα specific | DUF576 | 22 | IGG C | 30 | + | Nguyen et al., 2015b |
| 43 | SAUSA300_2429 | Tandem lpp | DUF576 | 22 | IGG C | 16 | 3* | |
| 44 | SAUSA300_2430 | Tandem lpp | DUF576 | 23 | IGA C | 29 | + | |
| 45 | SAUSA300_0100 | Tandem lpp/Conserved staphylococcal antigen 1A (Csa1A) | DUF576 | 24 | TAG C | 28 | + | Schluepen et al., 2013 |
| 46 | SAUSA300_0101 | Tandem lpp | DUF576 | 24 | TAG C | 28 | + | |
| 47 | SAUSA300_0102 | Tandem lpp | DUF576 | 24 | TAG C | 28 | + | |
| 48 | SAUSA300_0103 | Tandem lpp | DUF576 | 23 | TAG C | 28 | + | |
| Unknown function | ||||||||
| 49 | SAUSA300_0079 | Unknown function | DUF1541 | 18 | LSA C | 20 | 17 | |
| 50 | SAUSA300_0372 | Unknown function | PepSY | 18 | LTA C | 21 | 17* | |
| 51 | SAUSA300_0377 | Unknown function | DUF1748 | 19 | LTG C | 23 | 14 | |
| 52 | SAUSA300_1492 | Unknown function | 16 | LAG C | 13 | 15* | ||
| 53 | SAUSA300_0992 | Cell-wall binding lipoprotein | YkyA, EzrA | 19 | LAG C | 23 | 13* | |
| 54 | SAUSA300_2403 | Unknown function | DUF1307 | 20 | LSA C | 17 | 12* | |
| 55 | SAUSA300_0724 | Unknown function | IncA, TarH | 19 | ISA C | 32 | 12 | |
| 56 | SAUSA300_2315 | Unknown function | PA26, IncA, CLN3 | 17 | LAA C | 23 | 11* | |
| 57 | SAUSA300_2614 | Unknown function | DUF_1980 | 20 | LYS C | 42 | 6 | |
| 58 | SAUSA300_0663 | Unknown function | PA26, IncA | 17 | LTG C | 15 | 5 | |
| 59 | SAUSA300_1106 | Unknown function | FAM176 | 18 | VAG C | 35 | 5* | |
| 60 | SAUSA300_0303 | Unknown function | DUF4467 | 17 | LAG C | 14 | 5* | |
| 61 | SAUSA300_1478 | Unknown function | DUF4467 | 17 | LSA C | 13 | 3* | |
| 62 | SAUSA300_1376 | Unknown function | DUF1672 | 17 | LSG C | 34 | 2* | |
| 63 | SAUSA300_1379 | Unknown function | DUF1672 | 17 | LSG C | 34 | 2* | |
| 64 | SAUSA300_1440 | Unknown function | DUF1672 | 17 | LGG C | 34 | 2* | |
| 65 | SAUSA300_1742 | Unknown function | 18 | LTA C | 23 | 2* | ||
| 66 | SAUSA300_1741 | Unknown function | ETRAMP, Myco_19_kDa | 18 | LTA C | 6 | + | |
| 67 | SAUSA300_0769 | Unknown function | DUF5067 | 17 | LGA C | 27 | + |
The number indicates the number of staphylococcal species in which the corresponding homologues gene/protein (more than 40% identity over the whole protein length) is present.
+S. aureus specific gene.
Staphylococcal specific gene.
The corresponding gene has been compared in 34 staphylococcal species and other genera in which genome sequence has been available. Abbreviations: 60KD_IMP, 60Kd inner membrane protein; ABC2_membrane_3, ABC-2 family transporter protein; CamS, CamS sex pheromone cAM373 precursor; CfAFP, Choristoneura fumiferana antifreeze protein (CfAFP); CLN3, CLN3 protein; DM13, Electron transfer DM13; COX2, Cytochrome C oxidase subunit II; DUF, Domain Unknown Function; ETRAMP, Malarial early transcribed membrane protein (ETRAMP); EzrA, Septation ring formation regulator, IncA, IncA protein; Myco_19_kDa, Mycobacterium 19 kDa lipoprotein antigen; NMT1_2, NMT1-like family; Nit_Regul_Hom, Uncharacterized protein, homolog of nitrogen regulatory protein PII; OATP, Organic Anion Transporter Polypeptide (OATP) family; OpuAC, Substrate binding domain of ABC-type glycine betaine transport system; PA26, PA26 p53-induced protein (sestrin); PBP_like_2, PBP superfamily domain; PepSY, Peptidase propeptide and YPEB domain; Peripla_BP_2, Periplasmic binding protein; Pfam, Protein Families; Phosphonate-bd, ABC transporter, phosphonate, periplasmic substrate-binding protein; Rotamase, PPIC-type PPIASE domain; SBP_bac, Bacterial extracellular solute-binding protein; SP, Signal Peptide; TarH, Tar ligand binding domain homolog; YkyA, Putative cell-wall binding lipoprotein; ZnuA, Zinc-uptake complex component A periplasmic; ZinT, (YodA) periplasmic lipocalin-like zinc-recruitment; Zip, ZIP Zinc transporter.