Bacterial cultures obtained from crushed dental calculus samples on chocolate agar [a-f] growth of Klebsiella sp.[a], Lactobacilli sp.[b], Staphylococcus sp. [c], Proteus sp. [d], Pseudomonas sp. [e], Viridans streptococci [f] and on Mac Conkey agar [g-l] showing growth E.coli [g], Klebsiella sp. [h,i], Lactobacilli sp. [j], Pseudomonas sp. [k], Staphyloccucus sp.[l].