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. 2016 Sep 13;6:32656. doi: 10.1038/srep32656

Figure 1. In vivo MPTP injection induces Drp1 translocation to mitochondria in ventral midbrain and striatum at both 1d and 2d after the first MPTP injection.

Figure 1

(A) mitochondrial fractions, and (B) cytosolic fractions. P110 treatment (1.5 mg/kg/day) blocks Drp1 translocation to mitochondria. (C) confocal imaging analysis confirms co-localization of Drp1 with Tom20 in TH positive DA neurons in SNc after MPTP treatment, which is blocked by P110 treatment (more than 20 DA neurons are quantified in each group, Scale Bar = 20 μm). (D) Total Drp1 levels are not affected by P110 treatment. Blots are cropped to show proteins of interest. Original blots are shown in Supplemental Fig. 1. All gels have been run under the same experimental conditions. Data are expressed as mean ± SEM. *p < 0.05 and **p < 0.01 MPTP versus saline group; ##p < 0.01, TAT control peptide vs P110 treatment, analyzed by two-way ANOVA. (n = 3–4 in each group).