Table 1. MF-RA for 20 amino acids.

RES	11	2	3	4	5	6	7	8	9	10
A2	1.32	1.33	1.3	1.87	1.02	1.93	1.62	1.74	1.34	0.91
a2	0.53	0.58	0.55	0.85	0.68	0.88	0.78	0.82	0.71	0.61
C	1.78	2.28	3.98	2.56	2.2	4.42	2.74	2.9	3.39	2.76
c	0.65	0.63	0.81	0.86	0.61	0.71	0.34	0.69	0.65	0.71
D	1.46	1.29	0.83	0.76	0.8	0.61	0.42	0.46	0.62	0.53
d	3.26	2.48	3.61	2.65	1.68	2.36	1.58	2.27	1.49	1.73
E	0.5	0.49	0.83	0.56	0.74	0.88	0.74	0.54	0.53	0.34
e	1.96	2.21	2.61	2.87	2.95	2.13	2.36	1.72	1.62	1.37
F	1.43	1.84	1.65	1.67	2.03	1.95	2.86	1.75	1.69	2.49
f	0.68	0.83	0.92	0.89	0.87	0.88	0.95	0.57	0.8	0.85
G	2.12	2.05	2.31	3.25	2.24	1.62	1.85	1.54	3.27	1.6
g	0.9	0.83	0.98	0.99	1.27	1.11	1.04	1.04	1.76	0.92
H	1.47	1.56	1.08	1.17	1.58	1.16	1.98	1.29	0.93	1.45
h	2.14	2.49	2.37	2.48	1.77	2.68	3.31	2.43	1.49	1.87
I	1.41	1.43	1.69	1.34	1.61	2.52	1.33	2.21	2.16	2.07
i	0.61	0.66	0.51	0.58	0.6	0.61	0.38	0.7	0.89	0.65
K	0.51	0.39	0.33	0.56	0.47	0.39	0.43	0.44	0.39	0.43
k	3.46	3.39	2.35	5.73	3.83	2.61	3.02	3.96	3.61	3.92
L	1.46	1.63	1.46	1.41	1.23	1.88	1.6	1.53	1.48	1.2
l	0.65	0.93	0.55	0.61	0.54	0.46	0.49	0.48	0.59	0.45
M	1.77	2.45	1.94	2.15	1.68	2.08	2.26	2.27	2.24	1.48
m	0.74	1.36	0.83	1.15	0.78	0.69	0.89	0.98	0.83	0.61
N	1.01	1.27	0.76	0.87	1.16	0.41	0.73	0.78	0.91	0.75
n	1.71	1.92	1.63	2.11	1.57	0.99	1.48	1.31	1.66	1.48
P	1.53	0.42	1.5	0.78	0.54	1.5	0.51	0.62	0.48	0.78
p	1.5	0.44	1.5	1.16	0.68	1.5	0.55	0.66	0.27	0.8
Q	0.53	0.57	0.46	0.58	0.85	0.46	0.75	0.68	0.5	0.63
q	1.45	1.98	1.17	1.96	1.88	1.09	1.56	1.47	1.47	1.7
R	0.71	0.66	0.78	0.68	0.97	0.86	1.08	0.93	0.87	1.03
r	2.88	2.71	3.05	3.74	2.59	2.39	2.75	2.43	2.52	2.94
S	0.79	0.84	1.47	0.92	1.31	1.11	1.51	1.05	0.94	0.99
s	1.13	0.84	1.53	1.36	1.13	1.08	1.24	1.07	0.95	1.2
T	0.78	0.68	0.68	0.54	0.96	0.9	1.46	1.03	0.68	1.42
t	0.89	1.03	0.73	1.05	1.12	0.82	1.13	1.03	0.9	1.4
V	1.06	1.05	0.9	1.14	1.47	1.92	1.47	1.8	1.1	2.03
v	0.48	0.53	0.37	0.57	0.67	0.6	0.57	0.67	0.58	0.67
W	1.77	1.9	1.71	2.03	2.59	1.69	3.89	3.8	2.75	3.12
w	1.6	1.28	1.33	1.6	1.44	0.98	1.25	1.2	1.4	1.2
Y	1.12	1.17	1.26	1.33	1.97	1.34	2.13	1.66	1.09	2.03
y	1.2	1.07	1.43	1.5	1.65	1.28	1.59	1.23	0.96	1.14
RES	11	12	13	14	15	16	17	18	19	204
A	0.82	1.43	0.77	1.58	1	0.81	2.47	0.32	0.91	1.54
a	0.63	0.84	0.45	0.86	0.75	1.25	1.01	0.56	0.72	0.89
C	3.08	2.01	3.73	2.96	3.3	1.5	4.62	3.11	2.54	3.07
c	0.72	0.96	0.75	1.29	0.58	1.5	0.63	2.82	0.71	1.35
D	0.76	0.58	0.52	0.57	0.31	2.7	1.12	3.55	0.83	0.7
d	2.16	1.08	1.92	1.87	0.6	0.91	2.3	2.62	1.88	1.41
E	0.45	0.57	0.31	2.11	0.35	1.5	1.33	0.63	0.44	0.4
e	1.72	2.6	1.69	4.78	0.93	1.5	2.64	1.01	1.96	0.77
F	2.94	1.73	2.75	2.51	2.9	3.38	3.45	1.56	1.44	1.85
f	0.87	0.83	1.01	1.31	1.52	1.87	1.03	1.3	0.74	0.89
G	1.88	3.96	2.79	1.26	1.45	0.96	1.33	0.34	1.62	1.51
g	1.07	2.37	1.32	0.69	1.29	0.98	0.73	0.49	0.95	1.48
H	1.59	0.97	1.45	2.74	3.65	1.5	2.73	6.98	1.27	1.22
h	1.75	1.19	2.18	2.65	1.72	1.5	3.05	3.33	1.98	3.64
I	2.23	1.19	1.86	1.07	0.68	1.5	5.84	1.2	1.02	0.9
i	0.65	0.6	0.64	0.39	0.24	1.5	1.87	0.94	0.65	0.55
K	0.54	0.32	0.54	0.42	0.44	1.5	1.18	0.38	0.38	0.81
k	4.73	3.27	5.08	8.1	3.5	1.5	9.31	2.11	3.11	3.6
L	1.26	1.36	1.42	0.3	0.72	4.16	2.28	1.43	0.89	0.76
l	0.45	0.68	0.53	0.16	0.41	2.5	0.6	0.49	0.57	0.52
M	2.02	1.59	2.38	1.94	0.68	1.5	2.2	1.11	0.6	0.89
m	0.78	0.79	0.91	1.45	0.67	1.5	0.63	0.7	0.81	0.36
N	1.01	1.28	1	0.3	0.54	1.5	1.28	0.56	1.08	0.5
n	1.63	2.74	1.99	0.68	0.87	1.5	1.08	1.21	1.45	0.63
P	0.54	1.5	0.51	1.5	0.52	1.5	1.5	1.5	0.47	0.33
p	0.81	1.5	1.15	1.5	0.43	1.5	1.5	1.5	0.87	5.51
Q	0.66	0.48	0.48	1.1	0.53	1.5	0.7	0.75	0.52	0.15
q	1.58	1.44	1.5	1.76	1.23	1.5	1.4	1.06	1.4	0.41
R	1.07	0.78	0.77	1.26	0.38	2.25	1.18	0.61	0.7	0.8
r	2.92	2.27	3.37	5.06	2.67	3.75	1.68	1.13	2.62	1.89
S	0.87	1.27	1.23	0.61	1.15	3.49	1.79	1.07	0.95	0.93
s	1.14	1.33	1.33	0.82	0.9	1.67	1.38	0.79	1.09	0.58
T	1.16	0.79	1.69	0.94	1.01	1.02	2.01	2.03	0.77	0.54
t	1.05	1.1	1.63	0.68	0.63	1.56	0.82	0.65	0.82	0.4
V	2.1	0.84	1.39	0.79	1.3	1.5	4.87	1.11	0.61	0.85
v	0.73	0.56	0.55	0.52	0.26	1.5	1.34	0.66	0.47	0.53
W	2.72	1.92	2.59	3.9	2.04	1.5	6.06	1.95	2.41	1.11
w	1.19	1.17	1.32	4.49	2.67	1.5	1.66	1.21	1.35	1.1
Y	2.18	1.92	1.89	2.59	2.32	1.35	2.4	2.31	1.64	0.85
y	1.11	1.33	1.45	2.09	2.05	0.62	1.05	1.13	1.35	0.69

¹Region in the Ramachandran plot.

²Capital letters indicate high-accessibility amino acids (relative SASA > 13.1%), whereas lowercase letters indicate low-accessibility amino acids (relativeSASA ≤ 13.1%).

³The backbone conformation is considered rare if fewer than 20 residues are located in the region, and the MF-RA value of rare-conformation amino acids is 1.5 based on Petock et al., who reported that these residues are likely to be located at the functional site³⁵.

⁴DISICL divided the Ramachandran plot into 19 regions, and the rest of the area in the Ramachandran plot was considered the 20^th region³⁶.