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. 2016 Sep 13;6:33272. doi: 10.1038/srep33272

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MCF-7 cells were treated with PPM1D siRNA or control siRNA for 48 h, and were solubilized with 1x sample buffer for (a) Western blotting or (b) Immunocytochemistry. (a) PPM1D proteins were analysed by using Western blotting with rabbit polyclonal anti-PPM1D. (b) The subcellular localizations of NPM and PPM1D. PPM1D siRNA- or control siRNA-treated MCF-7 cells were fixed and stained with rabbit polyclonal anti-PPM1D and mouse monoclonal anti-NPM. (c) The percentage of cells with indicated nucleoli in PPM1D siRNA- or control siRNA-treated MCF-7 cells are shown. For quantitative analysis of the nucleolar number, stained-NPM was used as an indicator of the nucleoli. Number of cells used in the analysis are shown in Table 1; from at least two independent experiments.