Figure 3. HSCCC chromatogram of the refined urine sample after SL administration.
Solvent system: MTBE–n-BuOH–ACN–H2O (10:30:11:49, v/v/v/v). Detection wavelength was 280 nm. Sample loading was 4.3 g of refined urine extract in 80 mL of lower phase. Rotation speed was 500 rpm. The temperature of separation columns was maintained at 25 oC, and the flow rate of the mobile phase was 8.0 mL/min. Peak 1: Umbelliferone glucuronide (M1); Peak 2: Scopoletin glucuronide (M2); Peak 3: Umbelliferone sulfate (M3); Peak 4: Scopoletin sulfate (M4).