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. 2016 Sep 13;6:33036. doi: 10.1038/srep33036

Figure 4.

Figure 4

UPLC-UV chromatogram of (A) rat urine after SL administration, (B) metabolite M1, (C) M2, (D) M3, and (E) M4 obtained by HSCCC separation. Column: Waters HSS C18 column (1.7 μm, 2.1 mm × 100 mm, Waters Corp.) with a VanGuardTM pre-column (HSS C18, 1.7 μm, 2.1 mm × 5 mm); mobile phase: 0.1% formic acid in water (a) and 0.1% formic acid in acetonitrile (b) using a gradient program of 3% (b) in 0–2 min, 3–15% (b) in 2–14 min, 15–20% (b) in 14–22 min and 20–45% (b) in 22–30 min; flow rate: 0.3 mL/min; detection wavelength: 280 nm.