Table 1. Kinetic parameters of StOFOR1, its mutants, and StOFOR2 for each substrate^a.

	Pyruvate		2-Oxoglutarate
	Km (mM)	Vmax (U/mg)	Km (mM)	Vmax (U/mg)
StOFOR1	0.32 ± 0.08	7.5 ± 1.0	2.1 ± 0.3	2.7 ± 0.4
StOFOR1 S41aA	0.49 ± 0.09	2.2 ± 0.2	3.2 ± 0.4	1.1 ± 0.1
StOFOR1 T349aL	0.51 ± 0.11	3.2 ± 0.4	2.3 ± 0.3	2.0 ± 0.2
StOFOR1 D468aA	—b	0.10 ± 0.03	—b	NDc
StOFOR1 K49bI	0.91 ± 0.12	2.1 ± 0.2	—b	NDc
StOFOR2	1.6 ± 0.3	7.0 ± 0.9	15 ± 2	1.4 ± 0.2

^a0.25 mM CoA was used.

^bNot determined.

^cNot detected.