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. 2016 Sep 13;6:33030. doi: 10.1038/srep33030

Figure 4. PAR-1 signaling leads to proliferation and extracellular matrix production MES13 cells.

Figure 4

(a) PAR-1 mRNA expression in MES13 cells, HK-2 cells and imPTECs after 48 h of stimulation with 5 mM glucose (LG) or 25 mM glucose (HG). TBP expression served as reference gene. (b) Proliferation of MES13 cells after 24 h of stimulation with 25 mM glucose with and without p1pal12 (5 uM). (c) Proliferation of MES13 cells after 24 h stimulation with thrombin (1 U/ml) or PAR1-AP (100 μM) in the presence of p1pal12 (5 μM) or DMSO. (d–f) Western blot (lower panel) and quantification (upper panel) of fibronectin levels in MES13 whole cell lysates after 24 h stimulation with 20 mM mannitol or 25 mM glucose (d), p1pal12 (e), and thrombin (1 U/ml) or PAR1-AP (100 μM), with and without p1pal12 pretreatment (f). Indicated is the mean ± SEM, n = 3 individual experiments. One-way ANOVA with Bonferroni post-hoc analysis and unpaired t-test were used *p < 0.05; **p < 0.01; ****p < 0.01.