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. 2016 Sep 13;6:33030. doi: 10.1038/srep33030

Figure 5. Thrombin induces proliferation and fibronectin production in a MEK-p38-PKC and MEK-p38-Src dependent manner.

Figure 5

(a) Proliferation of MES13 cells after 24 h thrombin stimulation (1 U/ml) in the presence of inhibitors of mTOR (rapomycin, 20 nM), PKC (Chelerythrine, 1 μM), MEK (U0126, 10 μM), p38 (SB203580, 10 μM), or Src (PP1, 200 nM). (b) Western blot of fibronectin levels in MES13 whole cell lysates after 24 h thrombin stimulation in the presence of inhibitors of mTOR (rapomycin, 20 nM), PKC (Chelerythrine, 1 μM), MEK (U0126, 10 μM), p38 (SB203580, 10 μM), Src (PP1, 200 nM), or (c) TGFβ (10 μM LY364947 and 10 μM SB341542). (d) Western blot of phospho-ERK1/2 levels in MES13 whole cell lysates after thrombin stimulation for the indicated time points. Indicated is the mean ± SEM, n = 3 individual experiments. One-way ANOVA with Bonferroni post-hoc analysis and unpaired t-test were used *p < 0.05; **p < 0.01.