Fig. 2.

Knockdown of lncRNA XIST expression inhibits gastric cancer cell growth, colony formation, migration and invasion in vitro. a Relative expression level of lncRNA XIST in different gastric cancer cell lines (*P < 0.05, **P < 0.001). b Real-time PCR confirmed the knockdown of lncRNA XIST in gastric cancer cell SGC7901 and AGS (*P < 0.05). c and d Knockdown of lncRNA XIST inhibited cell proliferation as indicated by CCK-8 assays in SGC7901 and AGS (One-way ANOVA test, *P < 0.05). e Knockdown of lncRNA XIST inhibited colony formation as demonstrated by colony formation assays in SGC7901 and AGS (*P < 0.05). f and g Knockdown of lncRNA XIST inhibited cell invasion and migration as indicated by transwell and wound healing assays (*P < 0.05). h Knockdown of lncRNA XIST increased the level of epithelial markers such as E-cadherin, α-catenin while reduced the level of mesenchymal markers such as Vimentin and Fibronectin in SGC7901 cells