Fig. 5.

The effect of insulin on ANGPTL3 level in diabetic subjects. Unadjusted correlation analysis between plasma insulin level and ANGPTL3 level in non-diabetic participants (a) and diabetic patients (b). The parallel lines defined the 90 % prediction band. C57BL/6 and db/db mice were received peritoneal injection of 10 % glucose at 10 μl/g of body weight. Blood glucose was determined (c). d Representative western blot of pAkt and Akt in normal hepatocytes stimulated with 100 nM insulin ex vivo. (E) Western blot showing the expression of pAkt and Akt in diabetic hepatocytes stimulated with 100 nM insulin in the presence or absence of 1 μM pAkt inhibitor. f Hepatocytes were isolated from diabetic db/db mice and stimulated with 100 nM insulin in the presence or absence of pAkt inhibitor VIII. Representative western blot of pAkt and totak Akt in diabetic hepatocytes ex vivo. f ANGPTL3 and GAPDH expression in HepG2 cells by western blot and quantification of ANGPTL3 expression when adjusted by GAPDH expression. g Hepatocytes isolated from db/db mice were treated with 100 nM insulin in the presence or absence of Akt inhibitor VIII. Representative western blot of ANGPTL3 and GAPDH. ANGPTL3 expression was quantified by adjusting with GAPDH expression. Db/db mice were injected with insulin 2 U/kg. Plasma insulin (h) and ANGPTL3 was quantified (i). ^§p < 0.0001; ^ǂp < 0.001; ^łp < 0.01