Fig. 3.

Knockdown of IRAK1 inhibited proliferation and induced apoptosis in HCC cells. a Western Blot analysis of IRAK1 in MHCC-LM3 and SMMU-7721 cell lines after IRAK1 knockdown. b The normalization expression of IRAK1/β-actin. c The proliferation curves of MHCC-LM3 and SMMU-7721 cell lines after IRAK1 knockdown. d Apoptosis analysis of MHCC-LM3 and SMMU-7721 cell lines with the stimulation of cisplatin (Cis, 25 μg/ml). e The nucleocytoplasmic separation analysis of SMMU-7721 cell lines under the stimulation of cisplatin (Cis, 25 μg/ml) or epirubicin (ADM, 5 μg/ml). f Western Blot analysis of p-IRAK1 (T209) after IRAK1 knocked down. NC: control cell lines; SiIRAK1-1: IRAK1 knockdown cell lines with s1 fragment sequence; SiIRAK1-2: IRAK1 knockdown cell lines with s2 fragment sequence; s1, s2: different shRNA fragments in si-IRAK1 lentivirus. * P < 0.05