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. 2016 Sep 13;16(1):68. doi: 10.1186/s12896-016-0298-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Vector maps of pFCF1 and pFCF2. a Polypeptide sequence immediately downstream of the bidirectional promoter region found in gBlock1. Start codon sequence: black, FLAG sequence: green, TEV cleavage sequence: blue. MCS region from pUT18C colored by codons with restriction endonuclease sites shown below sequence. b pFCF1 vector map. Light blue bar indicates the promoter region. Unique restriction endonuclease sites shown in bold. FLAG: FLAG tag sequence; TEV: TEV cleavage site; TcR: tetracycline resistance gene; p15A ori: origin of replication. c pFCF2 vector map. Light blue bar indicates the promoter region. Unique restriction endonuclease sites shown in bold. HA: HA tag sequence; NeoR/KanR: neomycin/kanamycin resistance gene