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. Author manuscript; available in PMC: 2016 Sep 13.
Published in final edited form as: J Control Release. 2013 Aug 23;172(1):179–189. doi: 10.1016/j.jconrel.2013.08.015

Figure 1. Expression and purification of TH biopolymer.

Figure 1

a) Amino acid sequence of the TH. The recombinant, protein-based TH is composed of three functional motifs including condensation, dissociation and endosomal disruption motif, which are genetically engineered in one single molecule. b) SDS-PAGE analysis of purified TH followed by coomassie staining. The purity of the TH was estimated to be greater than 95%. c) Western blot analysis with mouse anti-Histidine x6 antibody to identify the TH.