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. 2016 Jul 27;20(10):1956–1965. doi: 10.1111/jcmm.12887

Figure 5.

Figure 5

Recruitment in p53‐activated/DNA complex following MNNG treatment depends on STAT1. (A) Western blot analysis for the indicated proteins at various times after treatment of the cells or not (NT) with MNNG (1 hr exposure; 10 μM). S15‐p53 and p53 were analysed in two individual western blots. This experiment was repeated four times. STAT1+/+ and STAT1−/− panels are cut from the same western blot. (B and C) STAT1+/+ and STAT1−/− cells were treated (+) or not (−) with 10 μM MNNG for 1 hr, in the presence (+) or not (−) of STI571 inhibitor (1 μM). 72 hrs following treatment, nuclear extracts were incubated with a biotinylated oligonucleotide containing the target canonical consensus sequence for activated p53. Complexes bound to activated‐p53 (S15) were analysed by western blotting using antibodies recognizing the indicated proteins. A total of 10 μg of the nuclear extracts were also loaded as a control of expression of the various proteins in STAT1+/+ cell extracts (input). A typical experiment out of two is shown.