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. 2016 Jul 27;20(10):1956–1965. doi: 10.1111/jcmm.12887

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of de novo expression of STAT1 alpha isoform on long‐term survival following MNNG exposure. (A) Total extracts from STAT1^+/+, STAT1^−/− and STAT1^−/−Tα cell lines were analysed by western blotting using antibodies recognizing the indicated proteins. A typical blot out of five is shown. (B) Clonogenic survival was analysed in the indicated cell lines at 10 days following a 1 hr exposure to the indicated doses of MNNG. A typical clonogenic assay representative of five independent experiments is shown. Percentage of colonies containing 50 cells or more was evaluated relative to untreated control. Statistical significance was determined by paired t‐test (*P < 0.05).