Figure 2.

Effect of TSA on TGFβ1‐induced Nox4 expression and H₂O₂ formation in endothelial cells. Pre‐treatment (1 hr) of HMECs with TSA (0.33 μM) suppressed TGFβ1 (10 ng/ml‐6 hrs)‐induced (A) Nox4 gene expression and (B) Nox4 protein expression as shown in a representative Western blot. (C) Basal and TGFβ1 (10 ng/ml)‐induced H₂O₂ generation was abolished in HMECs pre‐treated with TSA. Similarly pre‐treatment of (D) mouse lung endothelial cells and (E) human dermal fibroblast cells with TSA (0.33 μM) also suppressed basal and TGFβ1 (10 ng/ml‐6 hrs)‐induced Nox4 gene expression. All data are mean ± S.E.M. from 4 to 6 independent experiments, *P < 0.05 from control without treatment; †P < 0.05 from treated cells with TGFβ1.