Figure 5.

Effect of Nox4 inhibition on H₂O₂ production in endothelial cells. Treatment of HMECs with Adv‐Nox4i 72 hrs suppressed (A) Nox4 gene and (B) Nox4 protein expression, as shown in a representative Western blot and (C) H₂O₂ generation. (D) Similarly, wild type (WT) mouse lung endothelial cells (MLECs) derived H₂O₂ generation was less compared to Nox4 knockout (Nox4 KO) MLECs. All data are mean ± S.E.M. from 3 to 4 independent experiments, *P < 0.05 from Adv‐GFP or WT control.