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. 2016 Jul 28;20(10):1999–2011. doi: 10.1111/jcmm.12899

Figure 1.

Figure 1

The combination of two antibodies is needed for efficient internalization of HER2. (A) OE19 cells were incubated in growth medium without mAbs (Untreated) or with mAb 4517, mAb 4384 or a combination of mAb 4517 and mAb 4384 (mAbmix) for 4 hrs, before the cells were fixed, permeabilized and immunostained using an antibody to the intracellular domain of HER2 (clone Z4881). (B) OE19 cells were incubated in growth medium containing the mAb mixture for 4 hrs. HER2 intracellular domain (HER2 i.c.) was localized as in (A), and the mAb mixture was localized using antibody to human IgG. Micrographs representative for three experiments are shown, scale bars: 10 μm.