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. 2016 Jul 22;20(10):1803–1812. doi: 10.1111/jcmm.12865

Figure 1.

Figure 1

Effect of LNT on apoptosis of INS‐1 cells exposed to STZ. (A) Cells were pre‐treated with the indicated concentration of LNT for 30 min. and then incubated for 24 hrs with or without 0.5 mM STZ. Cell apoptosis was detected using flow cytometric assay with Annexin V‐FITC and PI‐staining. (B) TUNEL staining of cellular apoptosis of INS‐1 cells treated as in A. The images of TUNEL positive cells were captured with a confocal laser scanning microscopy (400× magnification). (C) Quantitative analysis of cellular apoptosis detected by flow cytometric measurements. (D) Quantitative analysis of TUNEL assay results. (E) Cell viability was further evaluated in INS‐1 cells treated as in A using an MTT assay. All the results are presented as the means ± S.E.M. for three independent experiments. **P < 0.01, ***P < 0.001 compared to the untreated control group; # P < 0.05, ## P < 0.01 compared to the STZ group.