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. 2016 Jul 22;20(10):1803–1812. doi: 10.1111/jcmm.12865

Figure 4.

Figure 4

Possible mechanism for the effects of LNT on STZ‐induced apoptosis and ROS production. (A) After pre‐treatment with LNT (200 μg/ml) or anisomycin (Am, 1 μM) for 30 min., INS‐1 cells were treated with the LNT (200 μg/ml) and STZ (0.5 mM) for 24 hrs. Cell apoptosis was detected using flow cytometric assay with Annexin V‐FITC and PI‐staining. (B) Quantitative analysis of cellular apoptosis detected by flow cytometric measurements. (C) INS‐1 cells were treated as in A. ROS production was analysed using Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence). The fluorescence signal was monitored at Ex/Em = 520/605 nm (cut‐off = 590 nm) with bottom read mode. **P < 0.01 compared to the untreated control group; # P < 0.05 compared to the STZ group; $ P < 0.05 compared to the STZ+Am group.