Skip to main content
. 2016 May 31;20(10):1861–1871. doi: 10.1111/jcmm.12876

Figure 5.

Figure 5

Beta cell destruction is NF‐κB/Bcl‐2 pathway dependent. (A) After pretreatment with or without PDTC (50 μM), an NF‐κB inhibitor, for 1 h, MIN6 cells were treated with a combination of IFN‐γ and TNF‐α for 12 h. The expression of cytoplasmic NF‐κB p65, Bcl‐xL, and Bax was determined by Western blot. Equal protein loading in all lanes was confirmed by probing the blots with anti‐β‐actin antibody. (B) After pretreatment with or without PDTC (50 μM) for 1 h, MIN6 cells were treated with or without the LIGHT and IFN‐γ combination for 48 h. Cell viability was measured by MTT assay. (C) Reverse expression of p65 and Bcl‐2 was observed in primary islets in NOD mice with (peri) insulitis by immunohistochemisty. [Left and right represent different fields in 2 NOD mice with (peri) insulitis]. Magnification, 200×. Data are expressed as mean ± SEM. *P < 0.05. Data are representative of two‐independent experiments.