Table 1. πSPIM resolution, anisotropy, and focal volumes.
| Mode | Objective(s) | NA | Resolution (FMWH)/nm | Anisotropy | Focal volume/fl | |
|---|---|---|---|---|---|---|
| type | x,y (theory) | z (theory) | (theory) | (theory) | ||
| widefield | PlanApoW25× | 1.1 | 274 (240) | 785 (801) | 0.35 (0.30) | 0.072 (0.057) |
| πSPIM | PlanApoW25× & ApoTIRF60× | 1.1/1.49* | 284 (212) | 339 (285) | 0.84 (0.74) | 0.057 (0.023) |
| widefield | PlanApoW60× | 1.27 | 250 (203) | 648 (510) | 0.39 (0.40) | 0.052 (0.027) |
Abbreviations: NA, numerical aperture; FWHM, full-width-at-half-maximum, anisotropy = FWHMx,y/FWHMz.
*The actual NA for light-sheet generation is limited by the access angle of the PlanApoW25× to ~0.71.