Figure 4. Vitamin D maintained tight junction function in TNBS-treated mice but not in oxazolone-treated mice.

(A) Western blot analyses of the colonic mucosal levels of tight junction proteins in different treatment groups on day 2. (B) Relative mRNA expression of tight junction proteins in different treatment groups on day 2 by real-time PCR. *P < 0.05, **P < 0.01 versus vehicle treatment (n = 6 in each group). (C) Confocal microscopic images of occludin staining in different treatment groups on day 2. Original magnification: 800×, bar = 25 μm; red staining represents the intercellular expression of occludin, which were counterstained with DAPI (blue). All electrophoresis was performed under the same condition, and gel images were cropped for concise presentation. The uncut images are provided in Supplementary Materials.