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. 2016 Sep 13;6:32889. doi: 10.1038/srep32889

Table 1. Mouse-specific primer sequences used for real-time PCR.

Primer name Forward (5′ - 3′) Reverse (3′ - 5′)
mouse TNF-α ATGAGCACAGAAAGCATGA AGTAGACAGAAGAGCGTGGT
mouse IFN-γ TTCTTCAGCAACAGCAAGGC TCAGCAGCGACTCCTTTTCC
mouse IL-2 TTGTGCTCCTTGTCAACAGC CTGGGGAGTTTCAGGTTCCT
mouse IL-4 AACGAGGTCACAGGAGAAGG TCTGCAGCTCCATGAGAACA
mouse IL-5 ACCGAGCTCTGTTGACAAG TCCTCGCCACACTTCTCTTT
mouse IL-6 CCTCTGGTCTTCTGGAGTACC ACTCCTTCTGTGACTCCAGC
mouse IL-10 ATAACTGCACCCACTTCCCA GGGCATCACTTCTACCAGGT
mouse IL-12 GATGACATGGTGAAGACGGC AGGCACAGGGTCATCATCAA
mouse IL-13 GCAGCATGGTATGGAGTGTG TGGCGAAACAGTTGCTTTGT
mouse IL-17 TCTCCACCGCAATGAAGACC CACACCCACCAGCATCTTCT
mouse IL-23 GCTGTGCCTAGGAGTAGCAG TGGCTGTTGTCCTTGAGTCC
mouse MCP-1 GCTCAGCCAGATGCAGTTAA TCTTGAGCTTGGTGACAAAAACT
mouse TGF-β1 CCTGCAAGACCATCGACATG TGTTGTACAAAGCGAGCACC
mouse Claudin-2 TATGTTGGTGCCAGCATTGT TCATGCCCACCACAGAGATA
mouse Occludin CCTCCAATGGCAAAGTGAAT CTCCCCACCTGTCGTGTAGT
mouse ZO-1 CCACCTCTGTCCAGCTCTTC CACCGGAGTGATGGTTTTCT
mouse B2M CGGCCTGTATGCTATCCAGA GGGTGAATTCAGTGTGAGCC