Table 1. Mouse-specific primer sequences used for real-time PCR.
Primer name | Forward (5′ - 3′) | Reverse (3′ - 5′) |
---|---|---|
mouse TNF-α | ATGAGCACAGAAAGCATGA | AGTAGACAGAAGAGCGTGGT |
mouse IFN-γ | TTCTTCAGCAACAGCAAGGC | TCAGCAGCGACTCCTTTTCC |
mouse IL-2 | TTGTGCTCCTTGTCAACAGC | CTGGGGAGTTTCAGGTTCCT |
mouse IL-4 | AACGAGGTCACAGGAGAAGG | TCTGCAGCTCCATGAGAACA |
mouse IL-5 | ACCGAGCTCTGTTGACAAG | TCCTCGCCACACTTCTCTTT |
mouse IL-6 | CCTCTGGTCTTCTGGAGTACC | ACTCCTTCTGTGACTCCAGC |
mouse IL-10 | ATAACTGCACCCACTTCCCA | GGGCATCACTTCTACCAGGT |
mouse IL-12 | GATGACATGGTGAAGACGGC | AGGCACAGGGTCATCATCAA |
mouse IL-13 | GCAGCATGGTATGGAGTGTG | TGGCGAAACAGTTGCTTTGT |
mouse IL-17 | TCTCCACCGCAATGAAGACC | CACACCCACCAGCATCTTCT |
mouse IL-23 | GCTGTGCCTAGGAGTAGCAG | TGGCTGTTGTCCTTGAGTCC |
mouse MCP-1 | GCTCAGCCAGATGCAGTTAA | TCTTGAGCTTGGTGACAAAAACT |
mouse TGF-β1 | CCTGCAAGACCATCGACATG | TGTTGTACAAAGCGAGCACC |
mouse Claudin-2 | TATGTTGGTGCCAGCATTGT | TCATGCCCACCACAGAGATA |
mouse Occludin | CCTCCAATGGCAAAGTGAAT | CTCCCCACCTGTCGTGTAGT |
mouse ZO-1 | CCACCTCTGTCCAGCTCTTC | CACCGGAGTGATGGTTTTCT |
mouse B2M | CGGCCTGTATGCTATCCAGA | GGGTGAATTCAGTGTGAGCC |