Figure 6. SPR measurements of the affinity of HypF-N type A and type B oligomers for immobilized DRMs.

(A) Representative immunoblot analysis of flotillin-1 distributions in 14 sucrose gradient fractions collected from the top (low density) to the bottom (high density) of the gradient tube of SH-SY5Y cells with basal GM1 content. A minor flotillin-1 reactivity appears in the two bottom fractions containing the major insoluble components of membranes. (B) Sensorgrams for type A (upper traces) or type B (lower traces) oligomers obtained by injecting the two oligomer types at the concentrations indicated into DRMs adsorbed onto SRP chips.