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. 2016 Sep 13;7:1353. doi: 10.3389/fpls.2016.01353

FIGURE 4.

FIGURE 4

Improved iron tolerance and the expression of vacuoleiron transporter genes in transgenic Arabidopsis. (A,B) Expression level of MEB2 in BnMEB2 over-expression in Arabidopsis wild type (WT, col-0) background (35S::BnMEB2/WT) and mutant meb2 (35S::BnMEB2/meb2) background transgenic lines. Seedlings were grown for 30 days and total RNAs were extracted for quantitative RT-PCR analysis. (C,D) The expression level of iron transporter genes in WT, meb2 and transgenic Arabidopsis. Twenty-day-old seedlings of WT, meb2, 35S::BnMEB2/WT and 35S::BnMEB2/meb2 which grown on MS medium were treated with FeSO4 (0.5 mM). AtActin was used as an internal control. (E) The comparison analysis of iron tolerance in Arabidopsis transgenic plants. The seedlings of WT, meb2, 35S::BnMEB2/WT and 35S::BnMEB2/meb2 were cultured on MS agar medium with a series of iron concentration (0.2 mM, 0.3 mM, 0.5 mM, 0.8 mM and 1 mM FeSO4) for 20 days.