FIGURE 5.

The detoxification ability of BnMEB2.(A) Phenotypes of WT, meb2 and transgenic Arabidopsis. Three-week-old seedlings of WT, meb2, 35S::BnMEB2/WT and 35S::BnMEB2/meb2 were grown in 0.5 mM FeSO₄ iron nutrient solution (H+Fe²⁺). (B–I) The iron storage in roots of WT, meb2 and transgenic Arabidopsis by representative Perls’ staining. The seedlings of WT, meb2, 35S::BnMEB2/WT and 35S::BnMEB2/meb2 were grown on MS medium (B–E) and MS medium added with 0.5 mM FeSO₄ (G–I) for 10 days. The arrow indicates the root hairs. The bar means 20 μm. (J,K) Iron contents and zinc contents in the seedlings of WT, meb2 and transgenic Arabidopsis. The seedlings of WT, meb2, 35S::BnMEB2/WT and 35S::BnMEB2/meb2 were grown in 0.5 mM FeSO₄ iron nutrient solution (H + Fe²⁺) for 30 days. Another set of the WT seedlings were grown in the normal Hoagland nutrient solution (H) for 30 days. Both sets incubated in the same environment conditions in a growth room. Statistical analysis were performed between WT under the H condition and each of four genotypes under the H + Fe²⁺ condition. Data are mean ± SD, ^∗P < 0.05, ^∗∗P < 0.01.