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. 2016 Sep 13;6:32651. doi: 10.1038/srep32651

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Standard plasma from 3 healthy volunteers was separated from EDTA- or citrate-anticoagulated blood (3,000 RPM/1,800x g; 10 minutes). EDTA tubes contained 10 mL of whole blood while citrate tubes contained 2.7 mL. Standard plasma was either exposed to a freeze/thaw cycle before a second centrifugation or processed without freezing, and select miRNAs were measured in the resulting PPP. Treatment groups were compared using a one-way ANOVA with a Tukey’s post-hoc test for multiple comparisons (** indicates P < 0.01, ***P < 0.001, ****P < 0.0001).